DNA Extraction and Purification
Zymo Research Corporation ZymoBIOMICS™ Microbial Community DNA Standard (200 ng)
One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem.
Zymo Research Corporation ZymoPURE™ P2 (Green) (410 ml)
ZymoPURE P2 (Blue) is designed to be used with our ZymoPURE Plasmid Purification Kits, which is the fastest and simplest method available to efficiently isolate transfection-grade plasmid DNA from E. coli.
Zymo Research Corporation CT Conversion Reagent, 1 tubes for 10 Conversions
CT Conversion Reagent-10 Conversions (1 Tube) for use with EZ DNA Methylation™ bisulfite conversion kits.
Zymo Research Corporation ZymoPURE P1 (Red), 150 ml
ZymoPURE™ P1 (Red) (150 ml). For use in ZymoPURE™ Plasmid Purification kits. Colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Zymo Research Corporation ZR Plasmid Miniprep™-Classic Kit (400 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin IIN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
Zymo Research Corporation Genomic DNA Clean & Concentrator™ Kit-10 (25 Preps)
There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied ZymoSpin™ Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Zymo Research Corporation EZ DNA Methylation-Gold™ Kit (50 Rxns)
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation Quick DNA Fecal/Soil Microbe MiniPrep™ Kit (50 Preps)
The kit can be used to successfully isolate DNA from tough-to-lyse Gram-positive and Gram-negative bacteria, fungi, algae, protozoa, etc. that inhabit fecal and soil samples. The procedure is easy and can be completed in as little as 15 minutes: fecal samples (150 mg each) or soil samples (250 mg each) are added directly to a ZR BashingBead™ Lysis Tube (0.1 & 0.5 mm) and rapidly and efficiently lysed by bead beating without the use of organic denaturants or proteinases. Zymo-Spin™ Technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that inhibit PCR. The DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, etc.
Zymo Research Corporation Zyppy™ Plasmid Miniprep Kit (800 Preps)
Our Zyppy™ Plasmid Miniprep Kit is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli. The plasmid DNA is of the highest quality, is endotoxin-free, and is well suited for use in bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications. An overview of the purification procedure is shown to the right.
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (rCALD1/820), CF740 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detec
Biochain Institute Inc CancerSeq Plus Paraffin Tissue Tumor Section: Lung, 5 Curls/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Occipital Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Tumor Tissue: Esophagus Tumor, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Arrhythmia, infarct: Heart Atrium, right, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Lung, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.